运输应激对山羊心的病理损伤及HSP27、HSP70和HSP90表达的影响

doi:10.11843/j.issn.0366-6964.2019.10.020

摘要/Abstract

摘要： 运用HE染色法、透射电镜技术、免疫组织化学、免疫印迹和ELISA等方法，探讨不同运输时间对山羊（Capra hircus）心的病理损伤及对热休克蛋白表达的影响，分析公路运输应激2、6 h条件下山羊心的显微结构、超微结构及HSP27、HSP70、HSP90的定位和表达量的变化情况。结果表明运输应激组山羊心肌纤维有程度不一的弥漫性颗粒变性，细胞内线粒体肿胀、破裂，间质水肿、出血、炎性细胞浸润，毛细血管扩张、充血，运输6 h组的病变更严重。HSP27、HSP70、HSP90主要在心肌细胞的细胞质中表达，HSP27和HSP90在细胞核中也有表达，三种蛋白在不同区域的心肌纤维和心肌细胞中分布不均。与对照组相比，运输应激2 h组山羊心HSP27蛋白表达量显著升高，运输应激6 h组HSP27表达量下降；与对照组相比，运输应激2和6 h组HSP70、HSP90蛋白表达量均显著或极显著升高。总之，运输应激山羊心组织细胞发生了病理损伤，心肌细胞急性变性十分明显，运输应激6 h组心肌细胞变性更严重，运输后HSP27、HSP70和HSP90蛋白表达量发生了明显变化，与心的应激损伤存在一定的关联。

Abstract: The effects of different transport time on pathological damage in the heart of goats (Capra hircus) were investigated with HE staining and transmission electron microscopy technique, and the expression of heat shock protein were analyzed with immunohistochemistry, Western blot and ELISA. The changes of microstructure, ultrastructure, localization and expression of HSP70, HSP27 and HSP90 in goat hearts under 2 and 6 hours of road transport stress were analyzed. The results showed that the myocardial fibers of goats in transport stress group exhibited diffuse granular degeneration of varying degrees, the intracellular mitochondria were swollen and broken, the edema, hemorrhage and inflammatory cell infiltration were observed in intercellular substance, capillaries were dilated and congested. And the lesion in the group transported for six hours was more serious. HSP70, HSP27 and HSP90 were mainly expressed in the cytoplasm of cardiac myocytes. HSP27 and HSP90 were also expressed in the nucleus of cardiac myocytes. This three proteins were unevenly distributed in myocardial fibers and myocardial cells of different regions. Compared with the control group, the expression levels of HSP27 protein in the heart of goats in group transported for two hours increased significantly, while the expression level of HSP27 after six-hour transportation stress decreased. Compared with the control group, the expression level of HSP70 and HSP90 protein in the groups transported for 2 and 6 hours increased significantly or increased extremely significantly. In conclusion, the pathological damage of cardiac tissue cells occurred in goats under transport stress, and the acute degeneration of cardiac cells was very obvious. The degeneration was more serious after six-hour transportation stress. The expression levels of HSP70, HSP27 and HSP90 proteins changed significantly after transport, which was related to the stress injury of heart.

中图分类号:

S852.3

郑文亚, 张雯苑, 刘犇, 杨雪, 肖鹏, 王安平, 崔燕. 运输应激对山羊心的病理损伤及HSP27、HSP70和HSP90表达的影响[J]. 畜牧兽医学报, 2019, 50(10): 2129-2138.

ZHENG Wenya, ZHANG Wenyuan, LIU Ben, YANG Xue, XIAO Peng, WANG Anping, CUI Yan. Effects of Transport Stress on Pathological Injury and Expression of HSP27, HSP70 and HSP90 of the Heart in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2129-2138.

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